Journal: PLoS ONE
Article Title: Co-Culture of Tumor Spheroids and Fibroblasts in a Collagen Matrix-Incorporated Microfluidic Chip Mimics Reciprocal Activation in Solid Tumor Microenvironment
doi: 10.1371/journal.pone.0159013
Figure Lengend Snippet: When co-cultured with fibroblasts, TS showed up-regulation of 7 angiogenesis-related proteins with 1.5-fold or greater changes (A) and down-regulation of 5 apoptosis-related proteins with greater than 30% changes (B). HT-29 TSs were grown for 6 days with or without fibroblasts in microfluidic channels and harvested for analysis using Proteome Profiler™ (see for details).
Article Snippet: The expression levels of proteins known for their roles in angiogenesis, apoptosis and cell motility were analyzed using the Proteome ProfilerTM (Human Angiogenesis Array kit, Human Apoptosis Antibody Array kit, R&D System, MN) according to the manufacturer's instruction.
Techniques: Cell Culture